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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

1,069 Views

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05:55 min

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February 10, 2023

DOI:

10.3791/64686-v

DOI:
10.3791/64686-v

•

05:55 min
February 10, 2023

•

3 Views
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1Department of Molecular Biosciences,The University of Texas at Austin, 2Department of Nutritional Sciences,The University of Texas at Austin

Summary

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CRISPR-Cas technologies have revolutionized the field of genome editing. However, finding and isolating the desired germline edit remains a major bottleneck. Therefore, this protocol describes a robust method for quickly screening F0 CRISPR-injected zebrafish sperm for germline edits using standard PCR, restriction digest, and gel electrophoresis techniques.

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Cite this Article

Voigt, B., Minowa, R., Gray, R. S. Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish. J. Vis. Exp. (192), e64686, doi:10.3791/64686 (2023).

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