Journal
/
Genetics
/
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

English

Automatically Generated

1,069 Views

•

05:55 min

•

February 10, 2023

DOI:

10.3791/64686-v

DOI:
10.3791/64686-v

•

05:55 min
February 10, 2023

•

3 Views
, ,
1Department of Molecular Biosciences,The University of Texas at Austin, 2Department of Nutritional Sciences,The University of Texas at Austin

Summary

Automatically generated

CRISPR-Cas technologies have revolutionized the field of genome editing. However, finding and isolating the desired germline edit remains a major bottleneck. Therefore, this protocol describes a robust method for quickly screening F0 CRISPR-injected zebrafish sperm for germline edits using standard PCR, restriction digest, and gel electrophoresis techniques.

Explore More Videos

Sperm ScreeningGermline EditsZebrafishCRISPRF0IndelsGenome EditsIn Vitro FertilizationPCRGel ElectrophoresisAnesthesiaCloacaCapillary ActionSodium HydroxideThermal CyclerTris-HClPCR ReactionAgarose Gel

Read Article

Cite this Article

Voigt, B., Minowa, R., Gray, R. S. Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish. J. Vis. Exp. (192), e64686, doi:10.3791/64686 (2023).

Download .ris file

Copy

Share Video

.

Copy