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Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

JoVE Journal
Genética

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JoVE Journal Genética

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

1,044 Views

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05:55 min

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February 10, 2023

DOI:

10.3791/64686-v

DOI:

10.3791/64686-v

•

05:55 min

February 10, 2023

•

11 Views

Brittney Voigt, Ryoko Minowa, Ryan S. Gray²

¹Department of Molecular Biosciences,The University of Texas at Austin, ²Department of Nutritional Sciences,The University of Texas at Austin

Tags

Sperm Screening Germline Edits Zebrafish CRISPR F0 Indels Genome Edits In Vitro Fertilization PCR Gel Electrophoresis Anesthesia Cloaca Capillary Action Sodium Hydroxide Thermal Cycler Tris-HCl PCR Reaction Agarose Gel

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