Journal
/
Developmental Biology
/
El sistema Zebrafish Tol2: un enfoque de transgénesis modular y flexible basado en pasarelas
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
Please note that all translations are automatically generated. Click here for the English version.

El sistema Zebrafish Tol2: un enfoque de transgénesis modular y flexible basado en pasarelas

español

Automatically Generated

2,316 Views

•

10:00 min

•

November 30, 2022

DOI:

10.3791/64679-v

DOI:
10.3791/64679-v

•

10:00 min
November 30, 2022

•

12 Views
, ,
1Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

Summary

Automatically generated

Este trabajo describe un protocolo para el sistema modular de transgénesis Tol2, un método de clonación basado en pasarela para crear e inyectar construcciones transgénicas en embriones de pez cebra.

Explore More Videos

ZebrafishTol2 SystemTransgenesisTransgenicFASD StudiesModularGateway-basedMRNALinearizationEndonucleaseSP6 EnzymeLithium Chloride Precipitation

Read Article

Cite this Article

Klem, J. R., Gray, R., Lovely, C. B. The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach. J. Vis. Exp. (189), e64679, doi:10.3791/64679 (2022).

Download .ris file

Copy

Share Video

.

Copy