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Das Zebrafish Tol2-System: Ein modularer und flexibler Gateway-basierter Transgenese-Ansatz
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Developmental Biology
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JoVE Journal Developmental Biology
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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Das Zebrafish Tol2-System: Ein modularer und flexibler Gateway-basierter Transgenese-Ansatz

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2,316 Views

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10:00 min

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November 30, 2022

DOI:

10.3791/64679-v

DOI:
10.3791/64679-v

•

10:00 min
November 30, 2022

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12 Views
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1Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

Summary

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Diese Arbeit beschreibt ein Protokoll für das modulare Tol2-Transgenese-System, eine Gateway-basierte Klonierungsmethode zur Herstellung und Injektion transgener Konstrukte in Zebrafischembryonen.

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Cite this Article

Klem, J. R., Gray, R., Lovely, C. B. The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach. J. Vis. Exp. (189), e64679, doi:10.3791/64679 (2022).

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