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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

2,316 Views

10:00 min

November 30, 2022

DOI:

10:00 min
November 30, 2022

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Summary

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This work describes a protocol for the modular Tol2 transgenesis system, a gateway-based cloning method to create and inject transgenic constructs into zebrafish embryos.

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