This work describes a protocol for the modular Tol2 transgenesis system, a gateway-based cloning method to create and inject transgenic constructs into zebrafish embryos.
Klem, J. R., Gray, R., Lovely, C. B. The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach. J. Vis. Exp. (189), e64679, doi:10.3791/64679 (2022).