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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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发育生物学
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JoVE 杂志 发育生物学
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

2,316 Views

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10:00 min

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November 30, 2022

DOI:

10.3791/64679-v

DOI:
10.3791/64679-v

•

10:00 min
November 30, 2022

•

12 Views
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1Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

Summary

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This work describes a protocol for the modular Tol2 transgenesis system, a gateway-based cloning method to create and inject transgenic constructs into zebrafish embryos.

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Klem, J. R., Gray, R., Lovely, C. B. The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach. J. Vis. Exp. (189), e64679, doi:10.3791/64679 (2022).

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