ジャーナル
/
発生生物学
/
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

DOI:
10.3791/64679-v

•

10:00 min

•

November 30, 2022

•

, ,
1Alcohol Research Center, Department of Biochemistry and Molecular Genetics,University of Louisville

章

  • 00:05Introduction
  • 00:53Transposase mRNA Preparation
  • 03:07Multisite Gateway Cloning to Create Entry Vectors for Transgenesis
  • 05:44Injection of the Transgene into the Embryos
  • 07:00Screening Embryos for Transgenic Insertion
  • 07:35Results: Transgenic Constructs of Zebrafish Transgenesis and Their Use in Ethanol Studies
  • 09:34Conclusion

概要

自動翻訳

自動翻訳

This work describes a protocol for the modular Tol2 transgenesis system, a gateway-based cloning method to create and inject transgenic constructs into zebrafish embryos.

タグ

ZebrafishTol2 SystemTransgenesisTransgenicFASD StudiesModularGateway-basedMRNALinearizationEndonucleaseSP6 EnzymeLithium Chloride Precipitation

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Microbead Implantation in the Zebrafish Embryo

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks

Precise Cellular Ablation Approach for Modeling Acute Kidney Injury in Developing Zebrafish

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Read Article