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Journal
/
Genetics
/
大
肠杆菌
中天然偶联质粒介导的水平基因转移检测
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal
Genetics
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in
E. coli
Please note that all translations are automatically generated.
Click here for the English version.
大
肠杆菌
中天然偶联质粒介导的水平基因转移检测
DOI:
10.3791/64523-v
•
06:56 min
•
March 24, 2023
•
Luis Mota-Bravo
,
Manel Camps
,
Iván Muñoz-Gutiérrez
,
Andrey Tatarenkov
,
Caison Warner
,
Isabel Suarez
,
Gerardo Cortés-Cortés
1
School of Biological Sciences
,
University of California Irvine
,
2
Department of Microbiology & Environmental Toxicology
,
University of California Santa Cruz
Chapters
00:05
Introduction
01:15
Conjugation
03:16
Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis
05:10
Results: Conjugative Plasmid Detection and Transfer
06:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
偶联通过动员质粒DNA跨两个不同细胞来介导水平基因转移，促进有益基因的传播。这项工作描述了一种广泛使用的高效检测共轭质粒转移的方法，该方法基于在共轭质粒、供体和受体中使用差异标记来检测转缀。
Tags
Horizontal Gene Transfer
Conjugative Plasmids
E. Coli
Antibiotic Resistance
Epidemiological Surveillance
Mating Assay
Donor
Recipient
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