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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
 

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Article DOI: 10.3791/64523-v • 06:56 min • March 24th, 2023
March 24th, 2023 •
1, 2, 1, 1, 2, 1, 2
1School of Biological Sciences, University of California Irvine, 2Department of Microbiology & Environmental Toxicology, University of California Santa Cruz

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Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

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Detection Horizontal Gene Transfer Natural Conjugative Plasmids E. Coli Antibiotic Resistant Genes Hospitals Commensals Aquatic Environments Metals Conjugation Efficiency Markers Selectable Markers Evolution Of Antibiotic Resistance Epidemiological Surveillance Drug-resistant Genes Caison Warner Pepper St.Clair Glycerol Stocks MacConkey Agar Mueller Hinton Broth Optical Density
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