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JoVE Journal Genetics
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
 

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Article DOI: 10.3791/64523-v • 06:56 min • March 24th, 2023
March 24th, 2023 •
1, 2, 1, 1, 2, 1, 2
1School of Biological Sciences, University of California Irvine, 2Department of Microbiology & Environmental Toxicology, University of California Santa Cruz

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Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

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Detection Horizontal Gene Transfer Natural Conjugative Plasmids E. Coli Antibiotic Resistant Genes Hospitals Commensals Aquatic Environments Metals Conjugation Efficiency Markers Selectable Markers Evolution Of Antibiotic Resistance Epidemiological Surveillance Drug-resistant Genes Caison Warner Pepper St.Clair Glycerol Stocks MacConkey Agar Mueller Hinton Broth Optical Density
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