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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
JoVE Journal
Genetica
This content is Free Access.
JoVE Journal Genetica
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

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06:56 min

March 24, 2023

DOI:

06:56 min
March 24, 2023

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Summary

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Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

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