JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
JA - 日本語
TR - Türkçe
Journal
/
Immunology and Infection
/
体外
纳米乳剂疫苗佐剂蛇腓磷素D的细胞活性评价
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
In Vitro
Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
Please note that all translations are automatically generated.
Click here for the English version.
体外
纳米乳剂疫苗佐剂蛇腓磷素D的细胞活性评价
DOI:
10.3791/64291-v
•
08:15 min
•
December 09, 2022
•
Xing Luo
,
Yanan Tong
,
Xiaoqiang Zeng
,
Yan Ye
,
Yun Yang
,
Zhen Song
,
Zelong Zhang
,
Haibo Li
,
Jining Gao
,
Xuhu Mao
,
Hao Zeng
,
Quanming Zou
,
Hongwu Sun
1
Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy
,
Third Military Medical University of Chinese PLA
,
2
Institute of Combined Injury of PLA, College of Military Preventive Medicine
,
Third Military Medical University of Chinese PLA
Chapters
00:05
Introduction
00:55
L929 Cytotoxicity Assay
01:51
Splenocyte Stimulation
04:02
Uptake by DCs
06:09
Macrophage Activation
06:57
Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
07:52
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议提供了评估纳米乳剂蛇腓苷D佐剂是否促进有效细胞免疫应答的详细方法。
Tags
In Vitro
Cellular Activity
Nanoemulsion
Vaccine Adjuvant
Ophiopogonin D
Cellular Response
Adjuvant Evaluation
L929 Cells
DMEM
Centrifugation
Mouse Spleen
Red Blood Cell Lysis
Cellular Delivery
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
迁移人T淋巴细胞分离,培养和分析<em在体外</em
所有流感的定量分析类型使用通用抗体的一种病毒Hemagglutinins和Neuraminidases,在简单的狭缝杂交分析
基于细胞的流式细胞仪检测,以测量细胞毒活性
限制性内切酶克隆方法,以评估<em>在体外</em> HIV-1 C亚型的Gag-MJ4嵌合病毒的复制能力
在
MyD88的介导的细胞免疫应答西尼罗河病毒变异株感染
体外
分析
宿主病原学反应及疫苗效果评价
体外ELISA测试评估狂犬病疫苗效力
鼻自组装纳米乳肿瘤疫苗体外和
体内
的
制备、特性、毒性及疗效评价
使用牛单核细胞来源的树突状细胞测定疫苗免疫原性
评估纳米乳剂佐剂疫苗对耐甲氧西林
金黄色葡萄球菌
(MRSA)感染的免疫反应
Read Article