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Immunology and Infection

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JoVE Journal Immunology and Infection
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
 

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Article DOI: 10.3791/64291-v • 08:15 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1, 1, 1, 1
1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy, Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine, Third Military Medical University of Chinese PLA

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The protocol presents detailed methods for evaluating whether the nanoemulsion ophiopogonin D adjuvant promotes effective cellular immune responses.

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Nanoemulsion Vaccine Adjuvant Ophiopogonin D In Vitro Cellular Activity Evaluation Experimental Approaches Cellular Response Efficacies Vaccine Adjuvants Extraction Methods BM Disease Toxicity Evaluation High Cellular Delivery Detailed Procedures Water Bath L929 Cells DMEM Centrifuge Culture Flask Primary Immunization Mice Alcohol
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