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In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
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JoVE Journal 免疫学と感染
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

DOI:
10.3791/64291-v

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08:15 min

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December 09, 2022

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, , , , , , , , , , , ,
1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

章

  • 00:05Introduction
  • 00:55L929 Cytotoxicity Assay
  • 01:51Splenocyte Stimulation
  • 04:02Uptake by DCs
  • 06:09Macrophage Activation
  • 06:57Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
  • 07:52Conclusion

概要

自動翻訳

自動翻訳

The protocol presents detailed methods for evaluating whether the nanoemulsion ophiopogonin D adjuvant promotes effective cellular immune responses.

タグ

In VitroCellular ActivityNanoemulsionVaccine AdjuvantOphiopogonin DCellular ResponseAdjuvant EvaluationL929 CellsDMEMCentrifugationMouse SpleenRed Blood Cell LysisCellular Delivery

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