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Journal
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Biochemistry
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高分子密集脂質膜上での1分子拡散と集合
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Please note that all translations are automatically generated.
Click here for the English version.
高分子密集脂質膜上での1分子拡散と集合
DOI:
10.3791/64243-v
•
10:43 min
•
July 19, 2022
•
Satyaghosh Maurya
,
Vishwesh Haricharan Rai
,
Aditya Upasani
,
Saurabh Umrao
2
,
Diksha Parwana
,
Rahul Roy
3
1
Department of Chemical Engineering
,
Indian Institute of Science
,
2
Holonyak Micro & Nanotechnology Lab
,
University of Illinois, Urbana-Champaign
,
3
Center for BioSystems Science and Engineering
,
Indian Institute of Science
Chapters
00:04
Introduction
00:53
Assembly of the Microfluidic Chamber
01:41
Making Supported Bilayers on Glass Substrate by Vesicle Fusion
04:22
Microscope Setup and Single-Particle Imaging Measurements
07:26
Image Acquisition for Counting Subunits in a Protein Assembly
08:40
Results: Analyzing the Binding of Cytolysin A (ClyA) Protein and Mobility of DNA Tracer
10:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、単一分子全反射蛍光(smTIRF)顕微鏡を使用して、人工混雑した脂質膜上の単一分子の結合、移動度、および組み立てを実行および分析するためのプロトコルが提示されます。
Tags
Single-molecule Imaging
Polymer-crowded Lipid Membranes
Supported Lipid Bilayers
Membrane Biomolecular Reactions
Diffusion
Assembly
POPC
DOPE-PEG(2000)
Small Unilamellar Vesicles
Sonication
Microfluidic Imaging Chambers
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