Journal
/
Biochimie
/
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Journal JoVE
Biochimie
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

2,039 Views

•

10:43 min

•

July 19, 2022

DOI:

10.3791/64243-v

DOI:
10.3791/64243-v

•

10:43 min
July 19, 2022

•

3 Views
, , , , ,
1Department of Chemical Engineering,Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab,University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering,Indian Institute of Science

Summary

Automatically generated

Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented.

Explore More Videos

Single-molecule ImagingPolymer-crowded Lipid MembranesSupported Lipid BilayersMembrane Biomolecular ReactionsDiffusionAssemblyPOPCDOPE-PEG(2000)Small Unilamellar VesiclesSonicationMicrofluidic Imaging Chambers

Read Article

Cite this Article

Maurya, S., Rai, V. H., Upasani, A., Umrao, S., Parwana, D., Roy, R. Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes. J. Vis. Exp. (185), e64243, doi:10.3791/64243 (2022).

Download .ris file

Copy

Share Video

.

Copy