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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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Biochemistry
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JoVE Journal Biochemistry
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

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7,957 Views

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07:49 min

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June 08, 2020

DOI:

10.3791/60950-v

DOI:
10.3791/60950-v

•

07:49 min
June 08, 2020

•

5 Views
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1School of Physics and Astronomy,University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College

Summary

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Conventional BODIPY conjugates can be used for live-cell single-molecule localization microscopy (SMLM) through exploitation of their transiently forming, red-shifted ground state dimers. We present an optimized SMLM protocol to track and resolve subcellular neutral lipids and fatty acids in living mammalian and yeast cells at the nanoscopic length scale.

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BODIPY ConjugatesSuper-resolution MicroscopySingle-molecule TrackingLive-cell ImagingLipid DropletsFatty AcidYeast CellsMammalian CellsEMCCD CameraLaser PowerMicroscope Optimization

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Adhikari, S., Banerjee, C., Moscatelli, J., Puchner, E. M. Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking. J. Vis. Exp. (160), e60950, doi:10.3791/60950 (2020).

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