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Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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Developmental Biology
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JoVE Journal Developmental Biology
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

English

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1,879 Views

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06:15 min

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July 06, 2022

DOI:

10.3791/64060-v

DOI:
10.3791/64060-v

•

06:15 min
July 06, 2022

•

8 Views
, , , , , ,
1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, 3Genus PLC

Summary

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Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.

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Adams, L., Liu, Y., Durrant, B., Ruggeri, E., Young, C., Krisher, R., Polejaeva, I. Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte. J. Vis. Exp. (185), e64060, doi:10.3791/64060 (2022).

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