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Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

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06:15 min

July 06, 2022

DOI:

06:15 min
July 06, 2022

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概要

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Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.

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