/
/
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
1,879 Views
•
06:15 min
•
July 06, 2022
DOI:
Summary
Automatically generated
Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.
Read Article
Cite this Article
Adams, L., Liu, Y., Durrant, B., Ruggeri, E., Young, C., Krisher, R., Polejaeva, I. Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte. J. Vis. Exp. (185), e64060, doi:10.3791/64060 (2022).
Copy