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JoVE Journal Developmental Biology
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
 

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 2, 2, 2, 3, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC
* These authors contributed equally

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Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.

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Keywords: Bisection Mitochondrial DNA Bovine Oocyte Cloning Heteroplasmy Centrifugation Zonae Pellucidae Pronase CBT20
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