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Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

DOI:
10.3791/64060-v

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06:15 min

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July 06, 2022

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, , , , , ,
1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, 3Genus PLC

Kapitel

  • 00:04Introduction
  • 00:47Centrifugation of the Oocytes
  • 01:51Preparation of the Oocyte for Bisection
  • 03:00Bisection of the Oocytes
  • 04:56Results: Successful and Unsuccessful qPCR Results and Boxplot from Mitochondria-Reduced and Mitochondria-Dense Ooplasts
  • 05:39Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to significantly reduce the mitochondrial DNA copy numbers in a bovine oocyte (P < 0.0001). This method utilizes centrifugation and bisection to substantially reduce oocyte mitochondria and may allow for an increased chance of development in the reconstructed interspecies somatic cell nuclear transfer embryos.

Tags

BisectionMitochondrial DNABovine OocyteCloningHeteroplasmyCentrifugationZonae PellucidaePronaseCBT20

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