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Chromosoomconformatie vastleggen over lengteschalen
JoVE Journal
Genetics
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JoVE Journal Genetics
Capturing Chromosome Conformation Across Length Scales
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Chromosoomconformatie vastleggen over lengteschalen

Nederlands

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3,053 Views

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10:15 min

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January 20, 2023

DOI:

10.3791/64001-v

DOI:
10.3791/64001-v

•

10:15 min
January 20, 2023

•

7 Views
, , ,
1Department of Systems Biology,University of Massachusetts Medical School, 2Howard Hughes Medical Institute

Summary

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Hi-C 3.0 is een verbeterd Hi-C protocol dat formaldehyde en disuccinimidylglutaraat crosslinkers combineert met een cocktail van DpnII en DdeI restrictie enzymen om de signaal-ruisverhouding en de resolutie van chromatine interactie detectie te verhogen.

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Cite this Article

Yang, L., Akgol Oksuz, B., Dekker, J., Gibcus, J. H. Capturing Chromosome Conformation Across Length Scales. J. Vis. Exp. (191), e64001, doi:10.3791/64001 (2023).

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