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Capturing Chromosome Conformation Across Length Scales
JoVE Journal
Genetics
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JoVE Journal Genetics
Capturing Chromosome Conformation Across Length Scales

Capturing Chromosome Conformation Across Length Scales

English

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3,002 Views

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10:15 min

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January 20, 2023

DOI:

10.3791/64001-v

DOI:
10.3791/64001-v

•

10:15 min
January 20, 2023

•

7 Views
, , ,
1Department of Systems Biology,University of Massachusetts Medical School, 2Howard Hughes Medical Institute

Summary

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Hi-C 3.0 is an improved Hi-C protocol that combines formaldehyde and disuccinimidyl glutarate crosslinkers with a cocktail of DpnII and DdeI restriction enzymes to increase the signal-to-noise ratio and the resolution of chromatin interaction detection.

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Cite this Article

Yang, L., Akgol Oksuz, B., Dekker, J., Gibcus, J. H. Capturing Chromosome Conformation Across Length Scales. J. Vis. Exp. (191), e64001, doi:10.3791/64001 (2023).

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