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発色団の存在下および非存在下におけるCu(II)とペプチド残基間の結合相互作用の定量化
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Biochemistry
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JoVE Journal Biochemistry
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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発色団の存在下および非存在下におけるCu(II)とペプチド残基間の結合相互作用の定量化

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2,218 Views

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11:38 min

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April 05, 2022

DOI:

10.3791/63668-v

DOI:
10.3791/63668-v

•

11:38 min
April 05, 2022

•

1 Views
, , ,
1Department of Chemistry,University of San Francisco, 2Department of Chemistry,University of California, Davis

Summary

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この記事では、電子吸収分光法と等温滴定熱量測定を使用して、ペプチドおよびタンパク質に結合するCu(II)の熱力学をプローブおよび定量することに焦点を当てます。

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Cite this Article

Choi, S., San Juan, J. A., Heffern, M. C., Stevenson, M. J. Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores. J. Vis. Exp. (182), e63668, doi:10.3791/63668 (2022).

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