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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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JoVE Journal 生化学
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:
10.3791/63668-v

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11:38 min

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April 05, 2022

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, , ,
1化学科,University of San Francisco, 2化学科,University of California, Davis

章

  • 00:04Introduction
  • 00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
  • 02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
  • 03:56Isothermal Titration Calorimetry
  • 09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
  • 10:48Conclusion

概要

自動翻訳

自動翻訳

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

タグ

Cu(II)PeptideMetal-peptide InteractionsElectronic Absorption SpectroscopyBufferCuvetteAbsorption SpectrumTitration

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