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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:

11:38 min

April 05, 2022

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Bölümler

  • 00:04Introduction
  • 00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
  • 02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
  • 03:56Isothermal Titration Calorimetry
  • 09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
  • 10:48Conclusion

Özet

Otomatik Çeviri

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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