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Kwantificeren van de bindingsinteracties tussen Cu (II) en peptideresiduen in de aan- en afwezigheid van chromoforen
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Biochemistry
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JoVE Journal Biochemistry
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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Kwantificeren van de bindingsinteracties tussen Cu (II) en peptideresiduen in de aan- en afwezigheid van chromoforen

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2,218 Views

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11:38 min

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April 05, 2022

DOI:

10.3791/63668-v

DOI:
10.3791/63668-v

•

11:38 min
April 05, 2022

•

1 Views
, , ,
1Department of Chemistry,University of San Francisco, 2Department of Chemistry,University of California, Davis

Summary

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Dit artikel richt zich op het gebruik van elektronische absorptiespectroscopie en isothermische titratiecalorimetrie om de thermodynamica van Cu (II) binding aan peptiden en eiwitten te onderzoeken en te kwantificeren.

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Cite this Article

Choi, S., San Juan, J. A., Heffern, M. C., Stevenson, M. J. Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores. J. Vis. Exp. (182), e63668, doi:10.3791/63668 (2022).

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