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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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生物化学
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JoVE 杂志 生物化学
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

2,218 Views

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11:38 min

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April 05, 2022

DOI:

10.3791/63668-v

DOI:
10.3791/63668-v

•

11:38 min
April 05, 2022

•

1 Views
, , ,
1Department of Chemistry,University of San Francisco, 2Department of Chemistry,University of California, Davis

Summary

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This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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Cite this Article

Choi, S., San Juan, J. A., Heffern, M. C., Stevenson, M. J. Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores. J. Vis. Exp. (182), e63668, doi:10.3791/63668 (2022).

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