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通过直接生物测定法鉴定海葵粗提取物中的溶血和磷脂酶活性
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
DOI:

12:12 min

March 29, 2022

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Chapters

  • 00:04Introduction
  • 01:29Organism Collection and Tissue Hydration
  • 02:26Toxin Release
  • 03:21Quantitation of Total Protein
  • 04:03Determine Polypeptide Venom Complexity and Protein Staining
  • 05:24Prepare Red Blood Cell Solution
  • 06:31Hemolysis Assay
  • 07:24Phospholipase Assay
  • 08:46Results: Nematocyst Discharge, Electrophoretic Profile of Sea Anemone Crude Extract, Hemolysis Assay in Erythrocytes from Sheep, and Phospholipase Assay
  • 11:01Conclusion

Summary

Automatic Translation

在这里,我们描述了一种从海葵中获得粗毒血清提取物并检测其溶血和磷脂酶活性的方案。

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