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簡単なバイオアッセイによる海アネモンからの粗抽出物中の溶血性およびホスホリパーゼ活性の同定
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Biochemistry
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JoVE Journal Biochemistry
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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簡単なバイオアッセイによる海アネモンからの粗抽出物中の溶血性およびホスホリパーゼ活性の同定

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12:12 min

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March 29, 2022

DOI:

10.3791/63630-v

DOI:
10.3791/63630-v

•

12:12 min
March 29, 2022

•

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1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

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Keyword Extraction:Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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Ramírez-Carreto, S., Salazar-García, S. I., Macías Martínez, G., Rodríguez-Almazán, C. Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays. J. Vis. Exp. (181), e63630, doi:10.3791/63630 (2022).

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