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Identificatie van hemolytische en fosfolipase-activiteit in ruwe extracten van zeeanemonen door Eenvoudige Bioassays
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Biochemistry
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JoVE Journal Biochemistry
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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Identificatie van hemolytische en fosfolipase-activiteit in ruwe extracten van zeeanemonen door Eenvoudige Bioassays

Nederlands

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2,421 Views

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12:12 min

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March 29, 2022

DOI:

10.3791/63630-v

DOI:
10.3791/63630-v

•

12:12 min
March 29, 2022

•

2 Views
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1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Summary

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Hier beschrijven we een protocol om ruw gifextract uit zeeanemoon te verkrijgen en de hemolytische en fosfolipase-activiteit ervan te detecteren.

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Keyword Extraction:Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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Cite this Article

Ramírez-Carreto, S., Salazar-García, S. I., Macías Martínez, G., Rodríguez-Almazán, C. Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays. J. Vis. Exp. (181), e63630, doi:10.3791/63630 (2022).

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