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Identification de l’activité hémolytique et phospholipase dans les extraits bruts d’anémones de mer par des essais biologiques simples
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Biochemistry
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JoVE Journal Biochemistry
Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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Identification de l’activité hémolytique et phospholipase dans les extraits bruts d’anémones de mer par des essais biologiques simples

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2,412 Views

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12:12 min

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March 29, 2022

DOI:

10.3791/63630-v

DOI:
10.3791/63630-v

•

12:12 min
March 29, 2022

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1Instituto de Biotecnología,Universidad Nacional Autónoma de México, 2Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

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Keyword Extraction:Hemolytic ActivityPhospholipase ActivitySea Anemone ToxinBioassaysCrude ExtractProteinHemolysisPhospholysisErythrocyteTemperatureLyophilizationCell LysisNematocyst DischargeCentrifugationBradford Colorimetric Assay

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Ramírez-Carreto, S., Salazar-García, S. I., Macías Martínez, G., Rodríguez-Almazán, C. Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays. J. Vis. Exp. (181), e63630, doi:10.3791/63630 (2022).

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