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通过非侵入性经鼻途径在免疫功能正常的小鼠大脑中移植人诱导的多能干细胞衍生小胶质细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
Please note that all translations are automatically generated.
Click here for the English version.
通过非侵入性经鼻途径在免疫功能正常的小鼠大脑中移植人诱导的多能干细胞衍生小胶质细胞
DOI:
10.3791/63574-v
•
05:35 min
•
May 31, 2022
•
Bijay Parajuli
2
,
Youichi Shinozaki
2
,
Eiji Shigetomi
2
,
Schuichi Koizumi
2
1
Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine
,
University of Yamanashi
,
2
GLIA Center
,
University of Yamanashi
Chapters
00:04
Introduciton
01:04
Preparation of iPSMG
02:01
Transnasal Transplantation
03:43
Results: Viability of iPSMG in the Cortex and the Hippocampus
04:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的方案允许在免疫功能正常的小鼠中通过经鼻途径将诱导多能干细胞衍生的人小胶质细胞(iPSMG)移植到大脑中。图中显示了用于制备和经鼻移植细胞以及施用细胞因子混合物以维持iPSMG的方法。
Tags
Induced Pluripotent Stem Cell-derived Microglia
Transnasal Transplantation
Immunocompetent Mice
CSF1 Receptor Antagonist PLX5622
Non-invasive
Neurodegenerative Diseases
Hyaluronidase
Cell Transplantation
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