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Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
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Neuroscience
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JoVE Journal Neuroscience
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

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8,919 Views

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00:06 min

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August 28, 2019

DOI:

10.3791/59305-v

DOI:
10.3791/59305-v

•

00:06 min
August 28, 2019

•

13 Views
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1Division of Biological Sciences,University of California, San Diego, 2Sanford Consortium for Regenerative Medicine

Summary

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This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. The procedure facilitates imaging-based assays of neurites, synapses, and late-expressing neuronal markers in a format compatible with light microscopy and high-content screening.

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Human Pluripotent Stem CellsNeuronsHigh-content ScreeningNeuritogenesisSynapse MaturationReplatingProteaseCell CultureDissociationTitrationCentrifugation

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Cite this Article

Calabrese, B., Powers, R. M., Slepian, A. J., Halpain, S. Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation. J. Vis. Exp. (150), e59305, doi:10.3791/59305 (2019).

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