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Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

DOI:
10.3791/57305-v

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08:35 min

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April 20, 2018

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, , ,
1Department of Neurosurgery,NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

Chapters

  • 00:05Title
  • 00:42Plate Fabrication
  • 02:02Indenter Block Alignment
  • 04:29Plate Stretching
  • 05:23Membrane Stretch Characterization and Cell Culture Injury
  • 06:58Results: Representative Cell Culture Injury Analyses
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

Tags

Human Induced Pluripotent Stem Cell-derived NeuronsHigh-speed Stretch Injury96-well FormatNeurotrauma ModelPhenotypic Drug DiscoveryAPTES CoatingSilicone MembranePlate Fabrication ClampStretching DeviceMotion ControlIn Vitro Neurotrauma

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