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Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

Transplantation of Human Induced Pluripotent Stem Cell-Derived Microglia in Immunocompetent Mice Brain via Non-Invasive Transnasal Route

DOI:
10.3791/63574-v

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05:35 min

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May 31, 2022

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, , ,
1Department of Neuropharmacology, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 2GLIA Center,University of Yamanashi

Chapitres

  • 00:04Introduciton
  • 01:04Preparation of iPSMG
  • 02:01Transnasal Transplantation
  • 03:43Results: Viability of iPSMG in the Cortex and the Hippocampus
  • 04:27Conclusion

Summary

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The protocol presented here allows the transplantation of induced pluripotent stem cell-derived human microglia (iPSMG) into the brain via a transnasal route in immunocompetent mice. The method for the preparation and transnasal transplantation of cells and the administration of cytokine mixture for the maintenance of iPSMG is shown.

Tags

Induced Pluripotent Stem Cell-derived MicrogliaTransnasal TransplantationImmunocompetent MiceCSF1 Receptor Antagonist PLX5622Non-invasiveNeurodegenerative DiseasesHyaluronidaseCell Transplantation

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