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Journal
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Biochemistry
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使用荧光团辅助碳水化合物电泳(FACE)方法测定糖原的葡聚糖链长分布
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光团辅助碳水化合物电泳(FACE)方法测定糖原的葡聚糖链长分布
DOI:
10.3791/63392-v
•
06:13 min
•
March 31, 2022
•
Léa Fermont
,
Nicolas Szydlowski
2
,
Christophe Colleoni
1
CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle
,
University of Lille
,
2
CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique
,
University of Lille
Chapters
00:05
Introduction
01:08
Incubation with Debranching Enzymes and Reductive Amination
02:37
FACE Analysis
03:32
Data Processing
04:08
Results: Determination of the Average Chain Length of Glycogen
05:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
在本方案中,荧光团辅助碳水化合物电泳(FACE)技术用于确定糖原的链长分布(CLD)和平均链长(ACL)。
Tags
Glucan Chain Length Distribution
Glycogen
Fluorophore-assisted Carbohydrate Electrophoresis (FACE)
Structural Organization
Water Solubility
Capillary Electrophoresis
Glycogen Metabolizing Enzymes
Branching Enzymes
Isoamylase
Pullulanase
Desalting
Sodium Cyanoborohydride
8-amino-1
3
6-pyrenetrisulfonic Acid
Reverse Polarity Electrophoresis
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