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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

20,129 Views

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15:27 min

•

April 17, 2017

DOI:

10.3791/55659-v

DOI:

10.3791/55659-v

•

15:27 min

April 17, 2017

•

4 Views

Danlin Yang¹, Ajit Singh², Helen Wu¹, Rachel Kroe-Barrett¹

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science,Columbia University

Summary

We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.

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