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Journal / Biochemistry / Determination of Glucan Chain Length Distribution …
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Biochemistry

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JoVE Journal Biochemistry
Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method
 

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

Article DOI: 10.3791/63392-v • 06:13 min • March 31st, 2022
March 31st, 2022 •
1, 1,2, 1
1CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle, University of Lille, 2CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique, University of Lille

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In the present protocol, the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) technique is used to determine the chain length distribution (CLD) and the average chain length (ACL) of glycogen.

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Glucan Chain Length Distribution Glycogen Fluorophore-assisted Carbohydrate Electrophoresis (FACE) Method Structural Organization Of Glycogen Particles Physical Chemical Properties Of Glycogen Particles Water Solubility APTS Molecule Fluorescence Intensity Capillary Electrophoresis Glycogen Metabolizing Enzymes Degree Of Polymerization Branching Enzymes Isoamylase Pullulanase Acetate Buffer PH 4.8 Incubation At 42 Degrees Celsius Centrifugation
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