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Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method
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Bioquímica
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JoVE Revista Bioquímica
Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

2,439 Views

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06:13 min

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March 31, 2022

DOI:

10.3791/63392-v

DOI:
10.3791/63392-v

•

06:13 min
March 31, 2022

•

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1CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle,University of Lille, 2CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique,University of Lille

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Glucan Chain Length DistributionGlycogenFluorophore-assisted Carbohydrate Electrophoresis (FACE)Structural OrganizationWater SolubilityCapillary ElectrophoresisGlycogen Metabolizing EnzymesBranching EnzymesIsoamylasePullulanaseDesaltingSodium Cyanoborohydride8-amino-136-pyrenetrisulfonic AcidReverse Polarity Electrophoresis

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Fermont, L., Szydlowski, N., Colleoni, C. Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method. J. Vis. Exp. (181), e63392, doi:10.3791/63392 (2022).

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