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使用2',7'-二氯荧光素双乙酸探针和流式细胞术在穆勒神经胶质细胞中定量活性氧
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
DOI:

14:25 min

May 13, 2022

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Chapters

  • 00:00Introduction
  • 01:37Performing the Assay
  • 04:21Cells Preparaion for Flow Cytometry
  • 06:57Data Acquisition in a Flow Cytometer
  • 09:00Analysis of the Acquired Data
  • 12:07Representative Results
  • 13:37Conclusion

Summary

Automatic Translation

在这里,我们提出了一种系统化,可访问和可重复的方案,以使用Müller神经胶质细胞(MGCs)中的2',7'-二氯荧光素二乙酸探针(DCFH-DA)检测细胞活性氧(ROS)。该方法用流式细胞仪量化总细胞ROS水平。该协议非常易于使用,适用且可重复。

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