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Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
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Neuroscience
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JoVE Journal Neuroscience
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

DOI:
10.3791/55488-v

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08:15 min

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May 22, 2017

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, , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2PhD Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, 3Life Science Center of Tsukuba Advanced Research Alliance (TARA),University of Tsukuba

Chapters

  • 00:05Title
  • 00:49Dissection
  • 03:52Imaging Vesicle Motility
  • 05:14Image Analysis
  • 06:21Results: Lysosome Motility in Neuronal Dendrites
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

The investigation of membrane trafficking is crucial for understanding neuronal functions. Here, we introduce a method for quantifying vesicle motility in neurons. This is a convenient method that can be adapted to the quantification of membrane trafficking in the nervous system.

Tags

Keywords: NeuronVesicle MotilityMembrane TraffickingFluorescent ProbesCell CultureCortical NeuronsEndosomeLysosome

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