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Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
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Biochemistry
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JoVE Journal Biochemistry
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver

DOI:
10.3791/63333-v

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12:07 min

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February 18, 2022

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, , , ,
1Research Institute for Biomedical Aging Research,University of Innsbruck

Chapters

  • 00:04Introduction
  • 00:55Preparation of Buffer, Tissue Sample, and Solution
  • 02:15Total Protein Extraction
  • 03:42SDS-PAGE and Western Blot Analysis
  • 04:39Ammonium Sulfate Precipitation
  • 06:28Ionic Exchange Chromatography with FPLC
  • 08:14Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
  • 09:32Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
  • 11:24Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to extract fumarylacetoacetate hydrolase domain-containing protein 1 (FAHD1) from swine kidney and mouse liver. The listed methods may be adapted to other proteins of interest and modified for other tissues.

Tags

Keywords: FAHD1 Protein PurificationProtein ExtractionAmmonium Sulfate PrecipitationCentrifugationFiltrationStorage Conditions

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