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Journal
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Biochemistry
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从猪肾和小鼠肝脏中提取和纯化FAHD1蛋白
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
Please note that all translations are automatically generated.
Click here for the English version.
从猪肾和小鼠肝脏中提取和纯化FAHD1蛋白
DOI:
10.3791/63333-v
•
12:07 min
•
February 18, 2022
•
Andreas Andric
,
Eva Wagner
,
Anne Heberle
,
Max Holzknecht
,
Alexander K. H. Weiss
1
Research Institute for Biomedical Aging Research
,
University of Innsbruck
Chapters
00:04
Introduction
00:55
Preparation of Buffer, Tissue Sample, and Solution
02:15
Total Protein Extraction
03:42
SDS-PAGE and Western Blot Analysis
04:39
Ammonium Sulfate Precipitation
06:28
Ionic Exchange Chromatography with FPLC
08:14
Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
09:32
Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
11:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何从猪肾和小鼠肝脏中提取富马酰乙酰乙酸水解酶结构域蛋白1(FAHD1)。所列的方法可以适应于其他感兴趣的蛋白质并针对其他组织进行修饰。
Tags
FAHD1 Protein Purification
Protein Extraction
Ammonium Sulfate Precipitation
Centrifugation
Filtration
Storage Conditions
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