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An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter
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Biochemistry
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JoVE Journal Biochemistry
An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter

English

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4,009 Views

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10:08 min

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May 21, 2021

DOI:

10.3791/62075-v

DOI:
10.3791/62075-v

•

10:08 min
May 21, 2021

•

7 Views
, , , , ,
1School of Life Sciences,University of Liverpool, 2Health Sciences Center,Texas Tech University

Summary

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A multi-column plate adapter allows chromatography columns to be interfaced with multi-well collection plates for parallel affinity or ion exchange purification providing an economical high throughput protein purification method. It can be used under gravity or vacuum yielding milligram quantities of protein via affordable instrumentation.

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Protein PurificationHigh-throughputMulti-column Plate AdapterMCPANickel Affinity ChromatographyIon Exchange ChromatographyParallel PurificationEconomicalVersatileFunctional ProteomicsBiophysical TechniquesVacuumGravityAutomation

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Cite this Article

Kineavy, F. B., Davies, A. A., Mitchell, M. R., Lay, D., Dominguez, M. J., Stollar, E. J. An Economical and Versatile High-Throughput Protein Purification System Using a Multi-Column Plate Adapter. J. Vis. Exp. (171), e62075, doi:10.3791/62075 (2021).

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