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2D 배양 시스템에서 인간 만능줄기세포를 췌장 베타세포 전구체로 분화
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
DOI:

10:12 min

December 16, 2021

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Chapters

  • 00:05Introduction
  • 01:22Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)
  • 02:10Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)
  • 04:19Generation of the Primitive Gut Tube from hPSCs (Stage 2)
  • 05:50Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors
  • 08:10Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs
  • 09:21Conclusion

Summary

Automatic Translation

본 프로토콜은 평면 단일층에서 인간 만능 줄기 세포 (hPSC)로부터 유래된 췌장 전구세포에서 PDX1 및 NKX6.1 전사 인자의 동시 발현을 증가시키는 향상된 방법을 기술한다. 이것은 신선한 매트릭스를 보충하고, 세포 밀도를 조작하고, 내배엽 세포를 해리시킴으로써 달성됩니다.

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