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JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
 

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

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Automatic Translation

The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.

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Keywords: Human Pluripotent Stem Cells Pancreatic Beta-cell Precursors 2D Culture System Cell Therapy Diabetes Pancreatic Development Definitive Endoderm Differentiation Immunocytochemistry CHIR-99021
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