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Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

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2,439 Views

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10:12 min

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December 16, 2021

DOI:

10.3791/63298-v

DOI:
10.3791/63298-v

•

10:12 min
December 16, 2021

•

14 Views
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1College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

Summary

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The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.

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Cite this Article

Memon, B., Abdelalim, E. M. Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System. J. Vis. Exp. (178), e63298, doi:10.3791/63298 (2021).

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